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CPLC Seminar: "Unraveling chromatin structure using single molecule force spectroscopy"

SpeakerJohn van Noort, Physics of Life Processes, Leiden University, The Netherlands
Date Feb 8, 2013
Time 2:00 pm  
Location 464 Loomis
Sponsor Center for the Physics of Living Cells
Contact Shawn McCormick
Event type Other
Views 1528

The compaction of eukaryotic DNA into chromatin has been implicated in the regulation of all processes involving DNA. However, the structure of chromatin remains poorly understood. This lack of structural information impedes a functional understanding of chromatin at the molecular level. Here I will discuss recent developments in single molecule force and torque spectroscopy techniques to study this higher order structure.

Using magnetic tweezers and reconstituted designer chromatin fibers, we show that such fibers stretch elastically up to three times their rest length. The stiffness is independent of the presence or absence of linker histones. At 3 pN an overstretching transition occurs that can be attributed to the rupture of nucleosome-nucleosome interactions.

For quantitative analysis of the compliance of the fibers we use a two-state model in which nucleosomes are stacked, as found in a folded fiber, or unstacked, representing the unfolded fiber. All force induced transitions up to 10 pN can be captured in this simple two-state model. Kinetic analysis of the rupture events suggests that stretching of the histone tails precedes the rupture of nucleosome-nucleosome interactions. Post-translational modifications of these histone tails, in particular acetylation of H4K16, have been implied to modulate nucleosome-nucleosome interactions. We measured the mechanical properties of fibers containing various post translational modifications. Changes in extension upon exertion of torsional stress clearly show that the chromatin fibers fold into a left-handed super-helix. Overall, by new single-molecule force spectroscopy techniques and quantitative analysis of the force-extension behavior of single chromatin fibers we resolved a structural and dynamic picture of chromatin folding.

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