Abstract: Despite numerous prevention and control strategies adopted by different countries over the past few decades, little progress has been made in reducing the prevalence of childhood anemia. A recent publication by the World Health Organization (WHO) analyzed the adoption of complementary strategies for the prevention and control of anemia in children 6 to 23 months of age, suggesting home fortification with multiple micronutrients in conjunction with basic healthcare actions. The aim of this ongoing study is to assess the effectiveness, acceptance and adherence of home fortification with vitamins and minerals for the prevention of anemia in infants attending at public healthcare centers. A multi-center study involving four Brazilian cities (Goinia, Olinda, Rio Branco and Porto Alegre) is proposed. The study design is a pragmatic cluster controlled trial involving health centers under the primary healthcare model (Traditional and Family Health Strategy). A sample size of 135 children in control and intervention groups, respectively, is envisaged. A total of 540 infants will be recruited from Rio Branco and Porto Alegre (Traditional healthcare model), 270 from Olinda (Family Health Strategy model) and 540 from Goinia (both Traditional and Family Health Strategy models), to give an overall sample of 1350 infants for the four cities. At study baseline, a control group of infants 12 to 14 months of age shall receive routine pediatric healthcare. In parallel, an intervention group of infants 6 to 8 months of age shall be submitted to an intervention involving home fortification as per WHO recommendations. After 6 months of the intervention, the effectiveness and impact of fortification will be analyzed by comparing intervention and control groups, both comprising infants 12 to 14 months of age. The experimental groups shall be compared overall and stratified by basic healthcare model.