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PRODID:-//University of Illinois//Web Services Calendar//EN
VERSION:2.0
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BEGIN:VEVENT
DTSTAMP:20120214T144201Z
DTSTART;TZID=America/Chicago:20100402T140000
DTEND;TZID=America/Chicago:20100402T140000
SUMMARY:"Molecular Engineering and Live Cell Imaging"
CREATED:20090821T130000Z
DESCRIPTION:Signaling molecules and their activities are well coordinated
  in space and time to regulate cellular functions in response to mechani
 cal and chemical microenvironment. Based on fluorescent resonance energy
  transfer (FRET)\, we have developed several genetically encoded biosens
 ors for detecting the spatiotemporal activities of signaling molecules\,
  including Src\, Rac1\, MT1-MMP\, and Calcium. With a Src biosensor\, lo
 cal mechanical stimulation induced by laser-tweezer-traction can be obse
 rved to cause a directional wave propagation of Src activation along the
  plasma membrane. Different Src activities can also be observed at diffe
 rent subcompartments when the Src biosensor is tethered on plasma membra
 ne in or outside of lipid raft. A Rac biosensor further revealed that th
 e Rac activity in cells constrained on micropatterned extracellular-matr
 ix surface is polarized with higher activity concentrated at the leading
  edge of migrating cells upon PDGF stimulation\, whereas Src activities 
 in these cells displayed global activation patterns without obvious pola
 rity. With a MT1-MMP biosensor\, epidermal growth factor (EGF) can be ob
 served to induce significant FRET changes in live cancer cells expressin
 g MT1-MMP\, but not in MT1-MMP-deficient cells. Active MT1-MMP was direc
 ted to the leading edge of migrating cells along micropatterned fibronec
 tin stripes\, via a process dependent upon an intact cytoskeletal networ
 k. Most recently\, our calcium biosensor revealed that there is a sponta
 neous Ca2+ oscillation in human mesenchymal stem cells (HMSCs) both insi
 de the cytoplasm and endoplasmic reticulum (ER). The substrate stiffness
  where HMSCs are cultured can significantly affect this Ca2+ oscillation
 \, in a fashion dependent on the RhoA signaling pathway. In summary\, ou
 r novel FRET biosensors in combination with tools in nanobiotechnology a
 nd biophotonics have made it possible to monitor key signaling cascades 
 in live cells with spatiotemporal characterization.
LAST-MODIFIED:20091112T140000Z
LOCATION:144 Loomis
CATEGORIES:CPLC Seminar
CONTACT:Shawn McCormick
ORGANIZER:smmccorm@illinois.edu
URL:http://illinois.edu/calendar/detail/1968?key=2000010120000101132016
UID:132016@illinois.edu
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