This is the first atomic structure of the ribosome solved by cryoEM on the U of I campus. “It’s breathtaking to see how each and every atom in this beautiful molecular machine arranged in three-dimension” said Dr. Jin.
Using the 3D atomic structure and biochemistry, Jin and team were able to decipher how a protein known as ArfA recognizes a stalled bacterial ribosome and recruits release factor RF2 to catalyze peptide release, a process that leads to rescuing the stalled ribosome in the bacterial cell.
Since bacterial and human cells employ completely different strategies to rescue stalled ribosomes, the rescue mechanism of bacteria is a drug target.
“This is also a collegial collaborative effort, our colleagues in the Beckman Institute, the research team led by Prof. Emad Tajkhorshid, provided us with powerful computational resources,” said Dr. Jin.
Read the full article here: http://www.nature.com/nature/journal/vaop/ncurrent/full/nature21053.html